Rneasy Mini Kit Protocol Pdf Download
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After the incubation, total RNA was extracted using the RNeasy mini kit and DNase protocol (Qiagen, #74104). mRNA contained in 2 μg total RNA was reverse transcribed using Transcriptor first strand cDNA synthesis kit (Roche, #4379012001). Amplification reaction assays contained SYBRGreen PCR Master Mix (Applied Biosystem, #4309155) and primers (IDT, 300 nM). RPS9 (40S ribosomal protein S9) was used as the reference gene for normalization and mRNA abundance was quantified using the threshold cycle method. 2b1af7f3a8